Table 3 Passing-Bablok regression analysis for method comparison between plasma p-tau assays
Assay 1 | Assay 2 | Transformation equation | Bias | |
|---|---|---|---|---|
Fixed | Proportional | |||
p-tau181 Simoa Quanterix | p-tau181 Lumipulse Fujirebio | y = −0.072 (−0.477–0.282) + 1.192 (1.005–1.418)x | ✔ | |
p-tau181 Simoa ADx | y = −20.841 (−29.991 – −15.429) + 25.021 (21.902–29.9)x | ✔ | ✔ | |
p-tau181 Lumipulse Fujirebio | p-tau181 Simoa Quanterix | y = 0.061 (−0.248–0.320) + 0.839 (0.706–0.99)x | ✔ | |
p-tau181 Simoa ADx | y = −21.121 (−30.339 – −14.599) + 21.958 (18.874–25.958)x | ✔ | ✔ | |
P-tau181 Simoa ADx | p-tau181 Simoa Quanterix | y = 0.833 (0.687–1.027) + 0.04 (0.033–0.046)x | ✔ | ✔ |
p-tau181 Lumipulse Fujirebio | y = 0.962 (0.761–1.193) + 0.046 (0.039–0.052)x | ✔ | ✔ | |
p-tau217 Simoa Janssen | p-tau217 Simoa Alzpath | y = −0.092 (−0.360–0.095) + 48.289 (42.952–53.143)x | ✔ | |
p-tau217 Simoa ADx | y = 0.001 (−0.007–0.009) + 1.681 (1.525–1.897)x | ✔ | ||
p-tau217 N-term Lumipulse ADx | y = 0.017 (0.011–0.023) + 2.557 (2.368–2.717)x | ✔ | ✔ | |
p-tau217 mid-region Lumipulse Fujirebio | y = −0.05 (−0.088 – −0.028) + 5.697 (5.359–6.226)x | ✔ | ✔ | |
p-tau217 Simoa Alzpath | p-tau217 Simoa Janssen | y = 0.002 (−0.002–0.007) + 0.021 (0.019–0.023)x | ✔ | |
p-tau217 Simoa ADx | y = 0.013 (−0.001–0.023) + 0.034 (0.031–0.039)x | ✔ | ||
p-tau217 N-term Lumipulse ADx | y = 0.026 (0.013–0.039) + 0.051 (0.047–0.055)x | ✔ | ✔ | |
p-tau217 mid-region Lumipulse Fujirebio | y = 0.004 (−0.032–0.025) + 0.106 (0.093–0.12)x | ✔ | ||
p-tau217 Simoa ADx | p-tau217 Simoa Janssen | y = −0.001 (−0.006–0.004) + 0.595 (0.533–0.652)x | ✔ | |
p-tau217 Simoa Alzpath | y = −0.369 (−0.729 – −0.021) + 29.262 (26.048–32.527)x | ✔ | ✔ | |
p-tau217 N-term Lumipulse ADx | y = 0.014 (0.000–0.025) + 1.465 (1.385–1.605)x | ✔ | ||
p-tau217 mid-region Lumipulse Fujirebio | y = −0.08 (−0.114 – −0.025) + 3.51 (3.074–3.984)x | ✔ | ✔ | |
p-tau217 N-term Lumipulse ADx | p-tau217 Simoa Janssen | y = −0.007 (−0.010 – −0.004) + 0.391 (0.366–0.421)x | ✔ | ✔ |
p-tau217 Simoa Alzpath | y = −0.509 (−0.770 – −0.239) + 19.479 (18.058–20.852)x | ✔ | ✔ | |
p-tau217 Simoa ADx | y = −0.009 (−0.018 – −0.001) + 0.683 (0.627–0.723)x | ✔ | ✔ | |
p-tau217 mid-region Lumipulse Fujirebio | y = −0.081 (−0.121 – −0.061) + 2.301 (2.089–2.493)x | ✔ | ✔ | |
p-tau217 mid-region Lumipulse Fujirebio | p-tau217 Simoa Janssen | y = 0.009 (0.005–0.015) + 0.176 (0.161–0.188)x | ✔ | ✔ |
p-tau217 Simoa Alzpath | y = −0.035 (−0.286–0.329) + 9.425 (8.219–10.951)x | ✔ | ||
p-tau217 Simoa ADx | y = 0.023 (0.009–0.029) + 0.285 (0.251–0.316)x | ✔ | ✔ | |
p-tau217 N-term Lumipulse ADx | y = 0.035 (0.021–0.049) + 0.435 (0.389–0.462)x | ✔ | ✔ | |