Table 2 Immunoassay specifications
Protein | Assay CV (median, SD, min–max) | Single-/multiplex | Vendor | Assay method | Product no. | Dilution (x-fold) |
|---|---|---|---|---|---|---|
FABP-3 | 3.4 ± 2.9 0.0–15.1 | 3-plex | Merck KGaA | Bead-based (Luminex Corp.) | HNS2MAG-95K | 5 |
Neurogranin | 2.6 ± 3.0 0.0–17.2 | |||||
Ferritin | 3.7 ± 4.5 0.0–19.8 | |||||
C1q | 2.2 ± 2.8 0.0–18.6 | 6-plex | HCMP2MAG-19K | 4000 | ||
C3 | 1.6 ± 2.4 0.0–15.8 | |||||
C4 | 1.6 ± 2.3 0.0–19.6 | |||||
Factor B | 2.0 ± 2.9 0.0–19.2 | |||||
Factor H | 1.7 ± 2.5 0.0–18.8 | |||||
C3b | 3.7 ± 4.3 0.0–19.8 | |||||
sAXL | 1.3 ± 1.8 0.0–16.8 | Singleplex | R&D Systems, Inc. | Colorimetric | DY154 | 250 |
sTyro3 | 2.0 ± 2.5 0.0–16.5 | Singleplex | DY850 | 6 | ||
YKL-40 | 1.2 ± 1.8 0.0–19.4 | Singleplex | DY2599 | 200 | ||
IL-6 | 1.9 ± 1.7 0.0–10.0 | Singleplex | Quanterix Corp. | Digital ELISA (SIMOA) | 101622 | 5 |
Il-18 | 2.1 ± 2.0 0.0–10.0 | Singleplex | 102700 | 500 | ||
CXCL10 | 2.2 ± 2.2 0.0–11.3 | 3-plex | Meso Scale Diagnostics, LLC. | Electrochemi-luminescence | U-PLEX K15047K and K15046K | 15 |
CCL2 | 1.7 ± 1.5 0.0–10.4 | |||||
MIF | 1.2 ± 1.6 0.0–18.2 | |||||
CRP | 1.8 ± 1.7 0.0–11.4 | Singleplex | K15198D | 1000 | ||
ApoE (total) | 3.5 ± 3.7 0.0–17.3 | Singleplex | F212I | 4000 | ||
sTREM2 | 4.2 ± 4.3 0.0–18.8 | Singleplex | Homebrew | 5 |