Fig. 2

Short-term feeding scheme searching for retention of Aβ in the gut tissue of D. rerio. (a) Schematic diagram of tissue extract regions, and estimated molecular weights of individual and combined components of the Aβ fusion protein expressed by transgenic D. melanogaster larvae. (b) Aβ (clone 6e10 antibody) western blotting of D. rerio gut tissue 24 hours after short-term feeding, 20 µg, n = 3 per condition. Aβ larvae = D. melanogaster larvae expressing Aβ fusion protein, 8 µg, n = 2. tdTomato larvae = D. melanogaster larvae expressing tdTomato, 8 µg, n = 2. Aβ peptide = human amyloid beta 1–42 peptide, 0.5 µg, n = 2. (c & d) Representative congo-red amyloid stained paraffin sections of (c) D. rerio gut 24 hours after feeding with larvae expressing tdTomato, n = 3, and (d) D. rerio gut 24 hours after feeding with larvae expressing Aβ₄₂ n = 3. Scale bars = 50 µm. (e) Detection of target proteins via MS in assorted tissues. Representative samples with the highest ProtScore, n = 3 per condition. ProtScore > 1.64 = < 1% Local false discovery rate. ProtScore > 0.47 = < 1% Global false discovery rate. ’-’ indicates no peptide evidence detected for analysis. Peptide confidence > 95%. Analysis performed in ProteinPilot software 5.03