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Fig. 7 | Alzheimer's Research & Therapy

Fig. 7

From: Intermittent hypoxia training enhances Aβ endocytosis by plaque associated microglia via VPS35-dependent TREM2 recycling in murine Alzheimer’s disease

Fig. 7

TFEB transcriptionally regulates Vps35 in BV2 cells. (A) A consensus TFEB-binding motif within the ChIP-seq peaks. (B) ChIP-PCR was performed to identify potential TFEB-binding elements in the Vps35 gene regulatory regions. IgG was used as a negative control. (C) ChIP-qPCR was conducted to detect the fold enrichment of the three identified CLEAR elements. (D) Sequences of ChIP-based CLEAR elements and mutants. (E) Dual luciferase activities of the constructs containing the promoter region of Vps35 with either intact (WT) or mutated (Mut) CLEAR elements were compared between shControl (NC) and shTFEB HEK293T cells. (F and G) Tfeb and Vps35 expressions in TA1-treated shTfeb BV2 cells were estimated with qRT-PCR. n = 3, *p < 0.05 and ***p < 0.001 by Student’s t-test (C) or two-way ANOVA (E to G). n.s. indicates no significant difference

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Alzheimer's Research & Therapy

ISSN: 1758-9193