Fig. 2

Differential expression of actin interacting proteins identified from WT and APP/PS1 mice. (A) GO analysis depicting functionally enriched pathways of significantly differentially expressed proteins (DEPs) in both WT and APP/PS1. (B) Volcano plot illustrating significantly differentially expressed proteins in both the groups. Proteins with a fold change > 1 are depicted in red, indicating increased expression, while proteins with a fold change <-1 are shown in green, indicating decreased expression, all corresponding to a significance level of p < 0.05. A total of 24 proteins were identified, with 22 proteins downregulated and 2 proteins upregulated. (C) Visualization of Gene Ontology (GO) term enrichment using the ClueGO / CluePedia plugin within Cytoscape. The network displays the connectivity of significant GO terms associated with differentially expressed proteins (DEPs) in both groups, with functional nodes and edges representing shared relationships based on a kappa score of 0.4. Only GO terms with a significance level of p ≤ 0.05 are included in the enrichment analysis. Differentially expressed proteins (DEPs) specific to each group are highlighted in red font. (D) Heatmap displaying the expression levels of significantly differentially expressed proteins from each sample in both groups