Fig. 3
From: Mitigation of synaptic and memory impairments via F-actin stabilization in Alzheimer’s disease
Memory recall and PSD-95 actin association restored by jasplakinolide in APP/PS1 mice. Representative immunoblot showing the association between PSD-95 and actin in WT and APP/PS1 mice at one-month (adolescent-ADL) (a, b), nine-month of age (middle-aged-MA) (c, d), and in F-actin fraction of synaptosomes in middle-aged APP/PS1 mice (e, f) (n = 8). G) Schematic of contextual fear conditioning memory paradigm. Jasplakinolide injection (h) rescued fear memory in middle-aged APP/PS1 mice (n = 8 (WT vehicle, APP/PS1 vehicle, APP/PS1 jas), n = 6 (WT jas), restored (i-j) synaptic F-actin levels (n = 8 (WT vehicle, APP/PS1 vehicle, APP/PS1 jas), n = 6 (WT jas), and (k-l) PSD-95 actin association (n = 8 (WT vehicle, APP/PS1 vehicle, APP/PS1 jas), n = 6 (WT jas). Statistical comparison between groups was conducted using either an unpaired two-tailed t-test (a-f) or a two-way ANOVA followed by Tukey’s post test. (h-l). Data are presented as mean ± SEM. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001