Fig. 7

Antibody-mediated inhibition of brain homogenate seeding measured using flow cytometry - Percentage FRET signal generated in Tau RD P301S FRET biosensor cells transfected with (A) L66 brain homogenate (5 µg/mL) or (B) AD brain homogenate (100 µg/mL total protein) following immunodepletion with the tau mAb panel in comparison to a negative control antibody. The results are shown by the order of their epitopes along the tau protein (from N- to C-termini) and colour-coded to reflect the different protein domains as indicated. N = 4 (L66) or 5 (AD) performed in duplicate, values are expressed as mean ± SEM, one-way ANOVA was performed with Dunnett’s post hoc test to compare the negative control antibody (B11, which targets non-specific protein) with anti-tau antibodies, n.s. = not significant, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001