Fig. 2
Increased Aβ induces neuroinflammation. Representative images of microglia labeled with Iba-1 (red), reactive astrocytes labeled with GFAP (magenta), and senile plaques labeled with Aβ (green) in hippocampus (A) and cortex (B), n = 3; (C) Aβ plaques recruited many activated microglia and astrocyte, n = 3; (D) MSD Mesoscale® analysis of soluble and insoluble Aβ38/40/42 levels in brain of 5×FAD mice, n = 6–7. (E) RT-PCR (IL-1α/TNF-α: n = 6) and Western-blot (MCH-2/MCP-1: n = 4). All values are expressed as mean ± SEM. Difference were analyzed by Two-tested Student’s t test and donated as follows: *p < 0.05, **p < 0.01, ***p < 0.001