Fig. 9
HLJDD alleviated neuroinflammation in 5×FAD mice. (A) Representative images of microglia labeled with Iba-1 (purple), reactive astrocytes labeled with GFAP (red), and senile plaques labeled with Aβ (green) in hippocampus, n = 4. (B) The levels of dentate gyrus, Aβ, Iba-1, and GFAP in the hippocampus were quantified by detecting the value of Integrated option density (IOD), n = 4. (C) MSD Mesoscale® analysis of soluble and insoluble Aβ38/40/42 levels in brain of 5×FAD mice, n = 6–9. (D) RT-PCR (IL-1α, TNF-α, INF-γ), n = 6. All the values were expressed as mean ± SEM. All data were analyzed by one-way ANOVA with Dunnett-test; *p < 0.05, ** p < 0.01, ***p < 0.001