Fig. 1

TNAP haploinsufficiency prevents disinhibited behavior and improves spatial memory in APP/PS1^+/− mice. (A) Breeding strategy: heterozygous TNAP mice (TNAP^+/−) were crossed with heterozygous APP/PS1 mice (APP/PS1^+/−) to obtain APP/PS1^+/−/TNAP^+/− offspring. (B) TNAP activity in the plasma from WT (n = 12), APP/PS1^+/− (n = 12), APP/PS1^+/−/TNAP^+/− (n = 8), and TNAP^+/− (n = 8) mice. Data are given as a percentage relative to WT mice, and the graphs show the distribution of values per group (median, IQR, maximum, and minimum values). (C) Timeline for Open Field (OF), Elevated Plus Maze (EPM), T-maze (TM), and Barnes Maze Test (BT or t-BT for training) behavioral assays performed by WT (n = 12), APP/PS1^+/− (n = 12), APP/PS1^+/−/TNAP^+/− (n = 8), and TNAP^+/− (n = 8) groups. When indicated, black square data points represent males and open square data points represent females. (D) Percentage of time in open arms in the EPM. (E) Percentage of time in the new arm during the TM. (F) Latency to escape hole across the training sessions of the Barnes test. (G) Time spent (left graph) and distance traveled (right graph) to find the escape hole in BT test day. (H) TNAP activity in hippocampal homogenates from WT (n = 12), APP/PS1^+/− (n = 12), APP/PS1^+/−/TNAP^+/− (n = 8)), and TNAP^+/− (n = 8) mice. Data are given as a percentage relative to WT mice, and the graphs show the distribution of values per group (median, IQR, maximum, and minimum values). ^#P ≤ 0.08; * P ≤ 0.05; ** P ≤ 0.01 or **** P ≤ 0.0001; using a one-way ANOVA followed by Tukey’s post hoc test