Fig. 1

Generation and characterization of hiPSCs from HS and sAD patients. A Schematic representation of the reprogramming process used to generate hiPSCs from HS and sAD fibroblasts. Human somatic cells from both HS (n = 5) and sAD (n = 5) donors were reprogrammed into hiPSCs using Sendai virus. B Table summarizing demographic data and clinical information of the donors used for generating HS and sAD hiPSCs. The table includes information on age, gender, ethnicity, APOE genotype, and Clinical Dementia Rating (CDR). (C) Representative of hiPSCs exhibiting pluripotent stem cell morphology (in contrast phase) and immunofluorescence images showing the expression of pluripotency markers in HS- and sAD-derived hiPSCs. Cells were stained for SOX2 (green), NANOG (green), OCT3/4 (green), SSEA-4 (red), and TRA-1–60 (red). DAPI (blue) was used to label nuclei. Both HS and sAD hiPSCs are positive for these pluripotency markers, confirming their stem cell identity. Scale bars: 50 µm