Fig. 4

sAD-hNs show altered neurite morphology compared to HS-hNs. A Cell body morphology and neurite length of HS- and sAD-hNs from DIV1 to DIV30 were measured by performing fluorescence-based NeuroTrack analysis (NT) in IncuCyte^® time-lapse microscopy system by exploiting EGFP-expression of hNs; Cell-body cluster area (in yellow), Neurites length (in red). B Dot plot graph showing cell body cluster area quantification of neuronal cells during 30 days of differentiation (n = 5/group); (B) Quantification of neurite length per neuron across different time points (DIV1, DIV14, DIV21, DIV30) shows a significant increase in neurite complexity in sAD neurons at DIV14, DIV21, and DIV30, while there is no significant difference at DIV1 (n = 5/group). C Analysis of cell bodies shows no significant differences between HS and sAD neurons across time points (n = 5/group). D Quantification of the cell viability at different time points, showing no significant difference between HS and sAD neurons (n = 5/group). E Representative camera lucida drawings from HS and sAD neurons at DIV30 with Sholl analysis. F–H Bar graphs showing differences in dendritic length (F), total number of bifurcating nodes (G) and total number of dendritic intersections (H) indicating decreased branching complexity in sAD neurons compared to HS neurons. Each dot in Figs B,C,D represents a triplicate of an experiment conducted on n = 5/group. Each dot in Figs F–H represents a single cell analysis conducted on n = 5/group. Data are presented as mean ± SEM, n.s. p > 0.05, *** p < 0.001, **** p < 0.0001 vs HS-hNs, assessed by assessed by 2way ANOVA-Sidak’s multiple comparisons