Fig. 5

Electrophysiological properties of neurons derived from HS and sAD patients. A Summary graphs of membrane potential values and (B) input resistance values recorded from HS- and sAD-hNs. C Representative traces showing current-evoked action potentials. D Frequency of action-potentials plotted against current pulse values. E Representative traces of spontaneous excitatory postsynaptic currents (sEPSCs) in HS (gray) and sAD (orange) neurons. F, G Quantification of sEPSC amplitude (F) shows no significant differences (n.s., p > 0.05), while frequency (G) is significantly decreased in sAD neurons compared to HS neurons (p < 0.01, n = 12 cells per group). H, I Analysis of rise time (H) and decay time (I) reveals no significant differences between HS and sAD neurons; n = 12 cells per group. Data are presented as mean ± SEM, n.s. p > 0.05, * p < 0.05 assessed by two tailed Student’s t test