Fig. 6

Neurons obtained from sAD-hiPSCs show impairments in synaptic protein expression. A Representative WB images of pre- and postsynaptic proteins in DIV30 HS- and sAD-hNs lysates; Densitometric analyses of presynaptic proteins: SYN-1, SYP and postsynaptic proteins: PSD95,GluA1; GAPDH was used as loading control; n = 5/group. C Immunofluorescence staining of synaptic markers SYN-1, SYP, PSD95, and GluA1 in HS and sAD neurons. Co-localization with EGFP (neuronal marker) is shown. sAD neurons display decreased synaptic marker expression. Scale bars: 50 µm. D Quantification of median fluorescence intensity (MFI) in HS and sAD neurons shows a significant reduction in SYN-1, SYP, PSD95, and GluA1 in sAD neurons compared to HS neurons; n = 5/group. Each dot represents the average of three independent experiments conducted on n = 5/group. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 vs HS-hNs, assessed by Mann Whitney Rank Sum test