Fig. 7

sAD-hNs display reduced phosphorylation levels of actin-modulating proteins LIMK1 and cofilin. A Representative WB images of p-PAK1 Ser204, p-LIMK1 Thr508 and p-Cof Ser3 levels in HS- and AD-hNs lysates at DIV30; (B) Bar graphs showing the densitometric analysis of p-PAK1 Ser204, p-LIMK1 Thr508 and p-Cof Ser3 levels in HS- and AD-hNs (n = 5/group). GAPDH was used as a loading control. norm. normalized protein levels. Each dot represents the average of three independent experiments conducted on n = 5/group. Data are presented as mean ± SEM, n.s. p > 0.05, * p < 0.05, ** p < 0.01 vs HS hNs, assessed by Mann Whitney Rank Sum test