Fig. 1

Images of hippocampus and entorhinal cortex from non-demented controls immunohistochemically stained against IRIS-1 (A) and IRIS-2 (B) show specific immunoreactivity in both cortex and the hippocampal subregions hilus and Cornu Ammonis 1 (CA1) Scale Bar = 400 μm. Images in (C-H) show representative images of hippocampal sections immunostained against IRIS-1 or IRIS-2 together with the microglia marker Iba-1, the astrocytic marker GFAP and the neuronal marker NeuN. Image in (C and D) shows a staining against IRIS-1 and IRIS-2 (purple in C and D, respectively) together with Iba-1 (green) and the nuclei staining DAPI (blue). Zoomed-in images of microglias in the yellow squares are shown to the right in (C and D). The images revealed no or very little colocalization between IRIS-1 and IRIS-2 and Iba-1. Images in (E and F) demonstrate GFAP positive astrocytes (green) co-stained against IRIS-1 and IRIS-2 (purple) and DAPI (blue). Zoomed-in images of astrocytes in the yellow squares are found to the right. The staining showed a moderate degree of colocalization between GFAP and IRIS-1 or IRIS-2 in astrocytes (indicated with arrows). Colocalization was also observed between IRIS-1 and IRIS-2 and the neuronal marker NeuN (visible as white puncta). Images, and zoomed-in neurons within yellow squares in (G and H) show localization of IRIS-1 and IRIS-2 (purple) within NeuN positive neurons (green) (indicated with arrows). The thickness of the Z-stack varies between 19.8 and 39.6 μm. Scale Bar = 20 μm