Fig. 6

(A) QRT-PCR analysis of mRNA levels for CDK5, MAPT, GSK3β, and ADAM17 in SH-SY5Y cells with and without CD59, IRIS-1, and IRIS-2 knockdown, using GAPDH as a reference gene (n = 3). (B) Western blotting assessments of separate representative repeats for Cdk5, total tau, and phosphorylated tau (AT8 antibody targeting phosphorylation at Ser 202, Thr 205) protein levels, respectively, in SH-SY5Y cells with and without CD59, IRIS-1, and IRIS-2 knockdown. Each representative membrane is grouped with its loading control (GAPDH) as indicated by the lines. Quantifications for each protein, based on eight biological repeats, are presented in (C, D and E), respectively. Statistical analyses for panel (A) were performed using two-way ANOVA and for panels (C, D and E) using two-tailed student T-test, with error bars indicating standard deviation (SD)