Fig. 2

Whole cell voltage clamp recording on GluN2C^WT and GluN2C^A1072V overexpressing neurons. A Representative traces of NMDA whole-cell total currents induced by prolonged puff application of NMDA (400 mM) in GluN2C^WT (black line, left) and GluN2C^A1072V (black line, right) overexpressing neurons. In green, responses of GluN2C^WT and GluN2C^A1072V overexpressing neurons exposed to NMDA delivery in presence of the GluNC/D blocker QNZ46 (QNZ) in the extracellular solution. B Analysis of NMDA-induced responses generated in untreated, GluN2C^WT and GluN2C^A1072V cells (one-way ANOVA, Tukey's multiple comparisons test, P = 0.035; each dot indicates the NMDA current generated in a single neuron). C Analysis of NMDA responses generated in GluN2C^WT vs GluN2C^A1072V cells in the presence of QNZ and expressed as a percentage of the control, i.e., NMDA responses recorded in a solution without QNZ (two-tailed unpaired t test, P = 0.002). Normal distribution was checked using the D'Agostino & Pearson normality test. Bar graphs show the mean ± s.e.m