Fig. 3

GluN2C^A1072V amino acid substitution in the GRIN2C gene promotes GluN2C surface expression and negatively affects the interaction of GluN2C with 14-3-3 scaffolding proteins. A Confocal images of surface (magenta) and total (green) GluN2C staining in GluN2C^WT (left)- or GluN2C^A1072V (right)-overexpressing neurons (yellow outline). Scale bar: 3 μm. B Bar graph representing the percentage of the control mean GluN2C surface/total ratio (two-tailed unpaired t test; t(53) = 2.693, p = 0.0095, n = 28–27/group from 4 independent experiments). C Schematic representation of the GluN2C protein structure. In the figure, the amino acid substitution (A1072V), the 14-3-3 binding motif, the PKB recognition motif and the PKB phosphorylation site are highlighted. D Confocal images of 14-3-3 (magenta) and total GluN2C (green) staining in GluN2C^WT (left)- or GluN2C^A1072V (right)-overexpressing neurons (yellow outline). Scale bar: 3 μm. E Bar graph representing the percentage of the control mean percentage of % of GluN2C colocalized with 14-3-3 (two-tailed unpaired t test; t(44) = 4.411, p < 0.0001; n = 23–23/group from 3 independent experiments). Bar graphs show the mean ± s.e.m. n = number of neurons