Fig. 9

APPPS1-hA7^ko microglia induce NLRP3 inflammasome expression in APPPS1 astrocytes in vitro. mRNA expression levels quantified by qPCR of genes involved in the priming and oligomerization of the NLRP3 inflammasome in APPPS1 + astrocytes treated with conditioned medium from APPPS1-hA7^ko mice for 24 h (A). Levels of selected proinflammatory/anti-inflammatory cytokines quantified by ELISA in APPPS1 astrocytes treated with conditioned medium from APPPS1-hA7^ko mice for 24 h (B). The data are presented as the means ± SDs (n = 3–5 independent cultures per experimental condition); significance was determined using one-way ANOVA followed by Tukey’s post hoc test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 vs. conditioned medium (+), LPS (+) group; #p ≤ 0.05 vs. conditioned medium (+), LPS (−) group)