Fig. 2

Alterations of microbiota in diversity and abundance following probiotic supplementation. A Fold change of Bifidobacterium Lactis, a main bacteria in Probiotic supplement, as quantified by qPCR. N: GFP + Ctrl Diet: 7 F/6 M; GFP + P/P Diet: 3 F/6 M; htauE14 + Ctrl Diet: 6 F/4 M; htauE14 + P/P Diet: 7 F/2M. B Chao1 analysis of Alpha Diversity levels. C Ordination plot of Beta Diversity index between AAV groups, using Bray-Curtis index distance method. There was no significant difference in Beta Diversity between GFP and htauE14 animals. D Ordination plot of Beta Diversity index between diet groups, using Bray-Curtis index distance method. A significant difference between community composition was found between probiotic and control diet groups. E A heat tree demonstrating bacterial abundance differences between diet groups. Labeled branches represent a significant abundance level between groups. Ctrl: control. P/P: probiotic + prebiobiotic. N: GFP + Ctrl Diet: 11 F; GFP + P/P Diet: 8 F; htauE14 + Ctrl Diet: 9 F; htauE14 + P/P Diet: 11 F. *p < 0.05, **p < 0.01