Fig. 3
The effects of htauE14 and probiotic supplement on tau phosphorylation in the LC and levels of associated GSK-3β activation in the hippocampus. A An example image of bilateral AAV infusion sites in the LC. B Co-expression of htauE14 (GFP; green) and dopamine β-hydroxylase (DBH; red) in LC neurons. Scale bars: 50 μm. C Representative blot of pTau S262 in LC tissue (left) and quantification of relative optical density (ROD; right). D Representative blot of pTau S356 in LC tissue (left) and quantification of relative optical density (ROD; right). N: GFP: 3 M; htauE14: 1 F/1M. E Representative blot of pGSK-3β across groups in hippocampal tissue. F RODs of pGSK-3β levels. G Comparison of pGSK-3β levels based on diet x sex interaction. H Representative blot of pGSK-3β across groups in hippocampal tissue. I RODs of GSK-3β levels. J Ratios of pGSK-3β/GSK-3β. N: GFP + Ctrl Diet: 4 F/4 M; GFP + P/P Diet: 4 F/4 M; htauE14 + Ctrl Diet: 4 F/4 M; htauE14 + P/P Diet: 4 F/4 M. *p < 0.05, **p < 0.01