Fig. 4

Certain GSMs display Aβ product line specificity. A Representative immunoblots of Aβ immunoprecipitated from conditioned medium of WT or KI MEF cells that were treated with the indicated GSMs (GSM-1 and RO5254601 at 2.5 µM, RO-02 and RO7019009 at 500 nM and BPN-15606 at 360 nM, respectively). B Ratios of secreted Aβ species (% of the sum of measured Aβ (37 + 38 + 40 + 42 + 43)) from WT or KI MEF cells that were treated with the depicted GSMs (n = 5–7). C Ratio of the secreted Aβ37 + 38/Aβ42 + 43 representing the processivity in both product lines. WT and KI MEF cells were treated with the depicted GSMs (n = 5–7). D Schematic overview of the Aβ product lines and their modulation by the various GSMs. Smaller red labelling indicates decreased production of the respective species while bigger green labelling represents increased production. Black and orange coloring indicates unchanged or only little changed Aβ production. Secreted Aβ species (A) were separated on Tris-Bicine urea gels and analysed by immunoblotting. The Aβ ratios (B, C) are shown together with those of the corresponding DMSO controls and presented as mean + SEM. Dashed red lines in (C) highlight the corresponding ratio of the DMSO vehicle-treated WT control. Missing data points are due to Aβ signals that could not be quantified