Fig. 4

Cytotoxic activity and degranulation activity of SNK01 against stimulated T cell. A Human T lymphocytes were cultured with (P/I) or without (Media) stimulation by PMA (5 ng/mL)/ionomycin (250 ng) (P/I) for 48 h. The cells were stained with PE-CD3, PerCP/Cy5.5-CD69, and APC-CD25 antibodies and then analyzed by flow cytometry. Contour plots represent percentages of the CD25⁺ CD69⁺ cells on CD3⁺ gated T cells. B Cytotoxic activity of SNK01 against T cells was assessed by flow cytometry. T cells labeled with CTV were activated either with (P/I) or without (Media) PMA and ionomycin, and then co-incubated with SNK01 cells at an E:T ratio of 1:1 for 4 h. C-D Degranulation activity and IFN-γ expression of SNK01 cells against T cells. T cells were activated either with (P/I) or without (Media) PMA and ionomycin and then mixed with SNK01 cells at an E:T ratio of 1:1. The expression of CD107a (C) or IFN-γ (D) in CD56 + population was assessed by flow cytometry after 5- or 3- hour incubation, respectively