Fig. 5

Characteristics of SNK01 cells. A The percentages of CD3⁻CD56⁺ NK cells, CD3 + T cells, CD20⁺ B cells, and CD14⁺ monocytes were analyzed flow cytometrically on freshly isolated cells from PBMC using CliniMACS CD56 microbeads (D0; before expansion) and expanded NK cells for 17–18 days of culture (D17-18). B The fold expansion of the total cell population after 17–18 days of culture (D17-18). C The cytotoxic activity of expanded NK cells against the K562 was measured via calcein-release assay at E:T ratios of 10:1 to 0.5:1 in triplicate. Dots and horizontal bars represent the mean value of each patient from 4 cultures for clinical trial and mean ± SD from all patients, respectively