Fig. 2

DOR activation attenuated Aβ generation and aggregation both in the cortex and hippocampus of APP/PS1 mice. (A) Effects of DOR and MOR on the level of soluble and insoluble Aβ42 in the cortex and hippocampus of APP/PS1 mouse brains. n = 3. Left panel: Cortex: ^***p = 0.0006, ^**p = 0.0053 or 0.0026 vs. WT. Hippocampus: ^****p < 0.0001, ^***p = 0.0002, ^**p = 0.0091 vs. WT; ^ΔΔp = 0.0014 vs. AD. Right panel: Cortex: ^*p = 0.0174 or 0.0345 vs. WT. Hippocampus: ^*****p < 0.0001, ^***p = 0.0009 vs. WT; ^ΔΔp = 0.0011 vs. AD. Two-way ANOVA was used to analyze the statistical significance. (B) Effects of DOR and MOR on the Aβ-positive plaques number in the cortex and hippocampus. n = 3 mice per group for statistical analysis. Scale bar = 100 μm in panel. ^*p = 0.0211, ^***p = 0.001 or 0.0009 vs. WT. One-way ANOVA was used to analyze statistical significance