Fig. 5

DOR and MOR played different role in modulating microglia activation. (A,B) Effects of DOR and MOR on cerebral microglia/macrophages infiltration in APP/PS1 mice analyzed by flow cytometry. n = 3. ^***p = 0.0003, ^****p˂0.0001 vs. WT. ^ΔΔp = 0.0018 vs. AD. One-way ANOVA was used to analyze statistical significance. (C,D) Representative images of the Iba1 fluorescent staining and the quantification of Iba1⁺ fluorescent intensity in the cortex and hippocampus of WT mice and APP/PS1 mice. n = 4 mice per group for statistical analysis. Scale bar = 100 μm in panel. Cortex: ^****p˂0.0001 vs. WT. ^ΔΔΔΔp < 0.0001 vs. AD. CA1: ^****p˂0.0001, ^***p = 0.0003 vs. WT. ^ΔΔΔΔp < 0.0001 vs. AD. DG: ^****p˂0.0001 vs. WT. ^ΔΔΔΔp < 0.0001 vs. AD. One-way ANOVA was used to analyze statistical significance. (E) Alternations in mRNA expression profile induced by DOR overexpression or MOR overexpression in normal BV2 cell line and Aβ1-42 exposed BV2 cell line. n = 3. The p value was presented in Supplementary 8. Unpaired t test was used to analyze the statistical significance