Fig. 6

DOR regulated HMGB1/NF-κB axis signaling pathway in AD mimicked BV2 cells. NC207: BV2 cells infected with lentivirus containing negative control 207 shRNA as the negative control for DOR; DOR KD: BV2 cells infected with lentivirus containing shRNA knocking down DOR; DOR OV: BV2 cells infected with lentivirus overexpressing DOR; NC794: BV2 cells infected with lentivirus containing negative control 794 shRNA as the negative control for MOR; MOR OV: BV2 cells infected with lentivirus overexpressing MOR. (A-C) Representative Western blot and quantification which show the effects of DOR and MOR activity alterations on HMGB1 and phosphorylation of NF-κB p65 protein level in BV2 cells. (B): ^**p = 0.0036 or 0.0023 or 0.0075, ^*p = 0.0274 or 0.0184 vs. C. ^ΔΔp = 0.0016 vs. A. n = 3. (C): ^*p = 0.0239 vs. C. ^ΔΔp = 0.0014 vs. A. n = 4. One-way ANOVA was used to analyze the statistical significance both in 6B and 6 C. (D-F) Representative Western blot and quantification of the effects of DOR and MOR on HMGB1 and phosphorylation of NF-κB p65 protein level in BV2 cells. n = 3. (E): Left panel: ^*p = 0.0336 vs. NC207. Right panel: ^**p = 0.0029 vs. NC794. (F): Left panel: ^*p = 0.0136 or 0.0145, vs. NC207. Right panel: ^***p = 0.0008 vs. NC794. The unpaired t test was used to analyze the statistical significance both in 6E and 6 F. (G) Representative Western blot and quantification of HMGB1 cytoplasmic translocation in BV2 cells. n = 3. ^*p = 0.0454, ^**p = 0.0063, ^***p = 0.0008 vs. C. ^ΔΔΔΔp˂0.0001, vs. A. One-way ANOVA was used to analyze statistical significance