Fig. 6
The effect of cornuside on LPS + Aβ1−42 induced NLRP3 inflammasome activation of BV2 cells and conditioned stimulation of HT22 cells. Data are expressed as the mean ± SEM (n = 3–6). A-D. Cytokine levels determined in the supernatant of cultured BV2 cells. E, G. Protein levels of NLRP3, ASC, Pro-caspase-1, Caspase-1, Pro-IL-1β, IL-1β and GSDMD-NT. F. The representative images of NLRP3 in BV2 cells (scale bar: 20 μm). H, I. Neuron survival rate and LDH levels in supernatant. J, K. Flow cytometric representation and statistical analysis of apoptosis in conditioned medium. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. control group; *p < 0.05, **p < 0.01 and ***p < 0.001 vs. model group