Fig. 2
CircAPP knockdown in microglia modulates microglial polarization, improves AD pathology and inhibits CLIC1 expression and channel activity. (A ~ G) The effects of circAPP knockdown on the expression of TNF-α, Pro- and cleaved IL-1β, CD16, Arg1, IL-10, Aβ42 and p-tau in the hippocampus of APP/PS1 mice were evaluated using Western blot assay, n = 4. (H) The effect of circAPP knockdown on Aβ phagocytosis of BV-2 cells was assessed using flow cytometry assay, n = 3. (I) Golgi staining assay was performed to assess the effect of circAPP knockdown on spine density in the hippocampus of APP/PS1 mice, n = 3 mice. (J ~ L) The effects of circAPP knockdown on total CLIC1 expression in Aβ-treated BV-2 cells (J) and the hippocampus of APP/PS1 mice (K) and CLIC1 membrane expression in Aβ-treated BV-2 cells (L) were evaluated using Western blot assay, n = 4. (M) The effect of circAPP knockdown on CLIC1 channel activity in Aβ-treated BV-2 cells was assessed using functional chloride channel assay, n = 6. All data in the figure are presented as mean ± SEM. Student’s t test (B ~ I, K) and one-way ANOVA (J, L, M) were used to assess statistically significant differences. *P < 0.05, **P < 0.01