Fig. 10

PBM regulates mitochondrial energy metabolism and microglial phagocytosis by reducing HK2 expression. A, D Representative images of ROS and quantitative fluorescence intensity of ROS in microglia cultured in vitro. Scale bar: 20 μm. B ATP levels in microglia in vitro. (C) RT-qPCR analysis of mRNA expression of glycolysis-related genes HK2 and TSPO in microglia. (E) Expression levels of pro-inflammatory cytokines TNF-α, IL-1β and anti-inflammatory cytokine IL-4 in the culture supernatant of microglia. F Flow cytometry was used to assess the ability of microglia to absorb Aβ. Experimental data are presented as the mean ± standard deviation. Compared with the CON group, *p < 0.05, **p < 0.01, ***p < 0.001; Compared with the LPS group, #p < 0.05, ##p < 0.01, ###p < 0.001