Fig. 1

Immunofluorescent staining of Aβ plaque and p-Tau Thr231 in the SCN, cortex and hippocampus of 2 and 10-month-old mice. The staining of Aβ plaque in the cortex and hippocampus of AD mice, scale bar: 100 μm (A). The area occupied by plaques in the hippocampus and cortex of AD mice (a). Data were the mean ± SEM values, n = 4–6 mice in each group. ***p < 0.001, by Student’s t-test. The p-Tau Thr231 staining in the mouse cortex and hippocampus, left scale bar: 50 μm, right scale bar: 20 μm (B-C). The p-Tau Thr231 staining in the SCN of mouse, scale bar: 50 μm (D). Integrated density of p-Tau Thr231 staining in the SCN, cortex and hippocampus were analyzed (b-d), n = 4 mice in each group. Data were the mean ± SEM values. *P < 0.05, by two-way ANOVA with Sidak’s multiple comparisons test