Fig. 1
Comprehensive tsRNA expression profile in APP/PS1 transgenic mice analyzed using tsRNA-Seq. (A) Distribution of tsRNA subtypes, showing an increased proportion of 5’-tiRNA and tRF-5c and a decreased proportion of tRF-5a in APP/PS1 transgenic mice cortex compared to controls (Control and APP/PS1: n = 3, biologically independent samples). (B) Volcano plot of tsRNA expression, highlighting 25 tsRNAs with significant differential expression (13 upregulated and 12 downregulated). (C) Specific information of differentially expressed tsRNAs, with 5’ tsRNAs marked in red. (D) qRT-PCR analysis of 5’ tsRNAs. tiRNAGly-CCC-2, tRFAla-AGC-3-M8, and tRFGly-TCC-1 showed consistent results with the sequencing data ( n = 6, biologically independent samples). Statistical significance was assessed using Student’s t-test (D) (p-value < 0.05; p values are indicated). All data are presented as means ± SEM. Each data point represents the average of 3 technical replicates