Fig. 2
Identification and validation of tsRNA-Target genes interactions and binding patterns. (A) The tsRNA-Target genes interaction network identifies EphA7 as a common target. (B) tRFAla-AGC-3-M8 binds to the EphA7 3’ UTR through an 8mer-1a pattern. 2D structure: The specific location of the binding site in the 3’ UTR (mRNA) or throughout a particular sequence. Local AU: AU weight distribution diagram around the seed sites, which influences site accessibility. The evaluation function slightly varies depending on the type of seed. The red bar indicates the position is A, with a higher number being more favorable. Position: The relative location of the site on the UTR, with proximity to the edges being more optimal. (C) Dual-Luciferase Reporter Assay results show that tRFAla-AGC-3-M8-mimic significantly decreases firefly luciferase activity (adjusted by renilla luciferase, normalized to NC mimic) in the EphA7-WT group (n = 9, biologically independent samples). Statistical significance was assessed using Student's t-test (p-value < 0.05; P values are indicated). All data are expressed as means ± SEM. Each data point represents the average of 3 technical replicates