Fig. 4

EphA7 expression in the cortex of APP/PS1 transgenic mice. (A) Brain slices from the APP/PS1 transgenic mice show Iba-1⁺ cell clusters (marked with white arrows) in the cortex, with higher expression of EphA7. Scale bar: 1000 μm. (B) Mean fluorescence intensity analysis of EphA7 expression in the cortex (n = 6, biologically independent samples). (C) Zoomed-in immunofluorescence image of the cortex. Scale bar: 20 μm. The fluorescence signal was manually traced to enhance visualization in schematic diagram (black: microglia; red: neuron). (D) Immunofluorescence analysis of Iba-1 (orange) and EphA7 (green) localization. Statistical significance was assessed using Student’s t-test (P values are indicated). All data are presented as means ± SEM. Each data point represents the experimental measure