Fig. 7
Western blot analysis of ERK1/2 and p70S6K phosphorylation in vivo and vitro model of AD. (A) Western blot analysis revealed a significant increase in the phosphorylation of ERK1/2 and p70S6K (T421/S424) in the APP/PS1 transgenic mice group compared to the control group (n = 6, biologically independent samples). (B) Phosphorylation of ERK1/2 and p70S6K (T421/S424) was increased in BV-2 cells treated with varying concentrations of Aβ25-35 (5 μM and 20 μM), n=6 samples/group. (C) HT22 cells treated with Aβ25-35 at concentrations of 10 μM and 20 μM exhibited elevated phosphorylation of ERK1/2 and p70S6K (T421/S424) (n = 6, biologically independent samples). Statistical significance was assessed using Student’s test (A) and one-way ANOVA (B and C) (P values are indicated). All Data are presented as means ± SEM. Each data point represents the average of 3 technical replicates