Fig. 8
Effects of tRFAla−AGC−3−M8 on ERK1/2-p70S6K pathway in BV-2 cells. (A, I) qRT-PCR analysis of tRFAla−AGC−3−M8 and EphA7 in BV-2 cells after transfection with tRFAla−AGC−3−M8-mimic or EphA7-siRNA (n = 6, biologically independent samples). (B-D, J-L) Western blot analysis of EphA7 and phosphorylation of ERK1/2 and p70S6K (T421/S424) in BV-2 cells (n = 6, biologically independent samples). (E, M) Representative light microscopy images of BV-2 cells morphological features; Scale bar: 75μm (overview), 20μm (insert). (F-H, N-P) Immunofluorescence localization an mean fluorescence intensity of iNOS in BV-2 cells (n = 6, biologically independent samples); Scale bar: 75μm (overview), 20μm (insert); Statistical significance was assessed using Student’s test (A and I) and one-way ANOVA (D, L, G and O) (P values are indicated). All data are presented as the means ± SEM. Each data point represents the average of 3 technical replicates