Fig. 9
Effects of tRFAla−AGC−3−M8 on ERK1/2-p70S6K pathway in HT22 cells. (A) qRT-PCR analysis of tRFAla−AGC−3−M8 and EphA7 in HT22 cells after transfected with tRFAla−AGC−3−M8-mimic or EphA7-siRNA (n = 6, biologically independent samples). (B-C) Western blot analysis of EphA7 expression and phosphorylation levels of ERK1/2 and p70S6K (T421/S424) (n = 6, biologically independent samples). (D) Representative image of HT22 morphological features; Scale bar: 75μm (overview), 20μm (insert). (E, F, G) Immunofluorescence localization and mean fluorescence intensity of p-tau (Red) in HT22 cells (n = 6, biologically independent samples), p-tau was labelled with solid white line in zoomed-in immunofluorescence image; Scale bar: 75μm (overview), 20μm (insert); Statistical significance was assessed using Student’s test (A) and one-way ANOVA (C and F) (P values are indicated). All data are presented as the means ± SEM. Each data point represents the average of 3 technical replicates