Fig. 2

AMFR associates with APP. A Endogenous AMFR and APP interaction in the hippocampus of 8-month APP/PS1 mice by Co-IP assays. The protein expression levels of AMFR and APP in the tissue lysates were confirmed. Data presented are representative of three separate experiments. B Endogenous AMFR and APP interaction in SH-SY5Y cells by Co-IP assays. The protein expression levels of AMFR and APP in the cell lysates were confirmed. Data presented are representative of three separate experiments. C Exogenous AMFR and APP interaction in SH-SY5Y cells transfected with empty vector or His-AMFR and empty vector or Flag-APP by Co-IP assays. The protein expression levels of AMFR and APP in the cell lysates were confirmed. Data presented are representative of three separate experiments. D Endogenous AMFR and APP interaction in HEK293T cells by Co-IP assays. The protein expression levels of AMFR and APP in the cell lysates were confirmed. Data presented are representative of three separate experiments. E Exogenous AMFR and APP interaction in HEK293T cells transfected with empty vector or His-AMFR and empty vector or Flag-APP by Co-IP assays. The protein expression levels of AMFR and APP in the cell lysates were confirmed. Data presented are representative of three separate experiments. F AMFR and APP co-localization in primary neurons of APP/PS1 embryonic mice, SH-SY5Y, HT22, and neuro-2a cells by confocal microscopy assays. AMFR (green), APP (red), cell nucleus (DAPI, blue), merge (AMFR + APP + DAPI); scale bars represent 50 μm. Data presented are representative of three separate experiments. G Endogenous AMFR and APP interaction in primary neurons of APP/PS1 embryonic mice by proximity ligation assay (PLA). The PLA signal is shown in red. Scale bars represent 1000 μm. Data presented are representative of three separate experiments.