Fig. 3

AMFR mediates proteasome-associated APP degradation. A AMFR and APP protein levels in SH-SY5Y-hAPP cells transfected with empty vector or His-AMFR. Data are shown as mean ± SEM from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001). B AMFR and APP protein levels in HEK293T-hAPP cells transfected with empty vector or His-AMFR. Data are shown as mean ± SEM from three independent experiments (**P < 0.01, ****P < 0.0001). C AMFR and APP protein levels in SH-SY5Y-hAPP cells transfected with siRNA-control or siRNA-AMFR. Data are shown as mean ± SEM from three independent experiments (*P < 0.05). D mRNA levels of AMFR and APP in SH-SY5Y-hAPP cells transfected with empty vector or His-AMFR by RT-qPCR assays. Data are shown as mean ± SEM from three independent experiments (ns, not significant, ****P < 0.0001). E AMFR mRNA levels in HEK293T-hAPP cells transfected with empty vector or His-AMFR by quantitative real-time PCR assays. Data are shown as mean ± SEM from three independent experiments (ns, not significant, **P < 0.01, ****P < 0.0001). F AMFR and APP protein levels in SH-SY5Y-hAPP cells transfected with empty vector or His-AMFR at different durations of cycloheximide administration. Data are shown as mean ± SEM from three independent experiments (*P < 0.05). G AMFR and APP protein levels in SH-SY5Y-hAPP cells transfected with empty vector or His-AMFR at DMSO or MG132 administration for 6 h. Data are shown as mean ± SEM from three independent experiments (*P < 0.05, ***P < 0.001, ****P < 0.0001). H AMFR and APP protein levels in SH-SY5Y-hAPP cells transfected with siRNA-control or siRNA-AMFR at DMSO or MG132 administration for 6 h. Data are shown as mean ± SEM from three independent experiments (*P < 0.05, **P < 0.01). I APP protein levels in HEK293T-hAPP cells transfected with His-AMFR at DMSO or MG132 administration for 6 h. Data are shown as mean ± SEM from three independent experiments (*P < 0.05, ***P < 0.001)