Fig. 3

Differential metabolites and metabolomics analysis. A-C: Volcano diagrams analysis of differential metabolites in EMCI/Control (A), LMCI/Control (B), and LMCI/EMCI (C). D: Multi-groups comparative analysis. The y-axis represented the names of metabolites, and the x-axis represented the average relative abundance of metabolites in different groups. E: Venn diagram of differential metabolisms in pairwise comparison, the number of overlapping parts represented the amounts of metabolites shared by multiple control groups, and the number of non-overlapping parts represented the amounts of metabolites unique to the corresponding groups. F-H: VIP diagrams were used to rank the differential contribution of metabolites to EMCI/Control (F), LMCI/Control (G), and LMCI/EMCI (H). The y-axis was the name of the metabolites, and the x-axis was the VIP value corresponding to the metabolites. The left and right color blocks on the right represented corresponding groups. Red and blue represented the high and low expression level of the metabolites in the group, respectively. I-K: KEGG pathway analyses of differential metabolites in EMCI vs. Control (I), LMCI vs. Control (J), and LMCI vs. EMCI (K)