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Table 1 Primers used in this study for RT-qPCR

From: Linking oxysterols and different stages of mild cognitive impairment: insights from gut metabolites and N6-methyladenosine

Primer

Forward sequence (5’‑3’)

Reverse sequence (5’‑3’)

m6A modifying enzymes

METTL3

CTATCTCCTGGCACTCGCAAGA

GCTTGAACCGTGCAACCACATC

METTL4

AGTGCCGACAGCATTGGTG

GGAGCAGAGGTATCATAGGAAGC

FTO

TTGGACGGTACAGATATGGAACATTTT

TCTTTTAGTTTCTTTGCCTTTGGGGAT

ALKBH5

CCAGCTATGCTTCAGATCGCCT

GGTTCTCTTCCTTGTCCATCTCC

Cognitive biomarkers

APP

ATGCTGCCCGGTTTGGCA

CTAGTTCTGCATCTGCTCAAAGAACTTG

BACE1

TACCAACCAGTCCTTCCGC

CTCCCATAACAGTGCCCGT

β-actin

AAGTGTGACGTTGACATCCG

GATCCACATCTGCTGGAAGG

  1. Abbreviations: METTL3, methyltransferase-like 3; METTL4, methyltransferase-like 4; FTO, fat mass and obesity-associated; ALKBH5, AlkB homolog 5; APP, amyloid precursor protein; BACE1, β-secretase
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Alzheimer's Research & Therapy

ISSN: 1758-9193