Fig. 6

Increased c-Fos expression in the DG of Tg2576 mice suggests enhanced neuronal activity. A. Micrographs of horizontal sections from WT (1) and Tg2576 (2) DG showing immunofluorescence (IF) for c-Fos protein (green), calretinin (red) and DAPI (blue) in the hilus, granule cell layer (GCL), molecular layer (ML) and CA3. The boxed areas are expanded below and show hilar cells expressing c-Fos (green) that were counted based on thresholding. Arrows point to hilar cells considered immunolabeled. B. Quantification of c-Fos IF showed increased expression in Tg2576 hilus and GCL. C. There were significantly more c-Fos + hilar cells in Tg2576 mice than WT mice. No significant differences were found for the number of GCs